电针“委中”穴对腰多裂肌损伤大鼠铁代谢的调节

目的观察多时间点大鼠多裂肌损伤后铁代谢相关蛋白的变化规律,探讨不同干预时间条件下电针"委中"穴对多裂肌损伤性腰痛的治疗作用。方法 SD大鼠按随机数字表法分为正常组、模型组、电针组,每组30只,各组再分为1 d、2 d、3 d、5 d、7 d 5个亚组,每个亚组6只。采用注射0.5%布比卡因(bupivacaine, BPVC)的方法造模,电针组电针双侧"委中"穴(1次/d),正常组、模型组不进行电针干预。分别于治疗的1 d、2 d、3 d、5 d、7 d取材。采用HE染色观察造模前后多裂肌形态变化;采用生化法检测多裂肌组织总铁含量变化;采用Western blot法检测铁蛋白重链(ferritin heavy chain1, FTH1)含量;采用Real-time PCR法检测多裂肌膜蛋白转铁蛋白受体1(transferrin receptor 1, Tfr1)、二价金属转运蛋白1(divalent metal transporter 1, DMT1)mRNA的表达。结果 HE染色结果显示,模型组相较于正常组可见肌纤维断裂、坏死并伴有炎性细胞浸润;与同时间点模型组相比,电针组受损肌纤维可见明显改善。生化法检测结果显示,与同时间点正常组相比,模型组、电针组总铁含量升高(P<0.01);Western blot、Real-time PCR检测结果提示模型组FTH1蛋白表达低于正常组(P<0.05或P<0.01),而Tfr1与DMT1 mRNA表达均高于正常组(P<0.05或P<0.01);与同时间点模型组相比,电针1 d、2 d、3 d、5 d组FTH1蛋白表达升高(P<0.05或P<0.01),而电针2 d、3 d、5 d、7 d组Tfr1 m RNA和电针1 d、2 d、3 d、5 d组DMT1 mRNA表达均降低(P<0.05或P<0.01);各模型组间比较发现,模型2 d组FTH1蛋白表达最低(P<0.05),而模型2 d、3 d组Tfr1 mRNA表达较高(P<0.05),模型3 d组DMT1 mRNA表达最高(P<0.05);各电针组比较结果显示,FTH1蛋白在电针2 d组最高(P<0.05),电针3 d、5 d、7 d组Tfr1 mRNA表达较低(P<0.05),电针3 d组DMT1 mRNA表达较高(P<0.05)。结论在BPVC致多裂肌损伤模型中,局部受损多裂肌发生铁代谢紊乱,且在急性损伤期较典型。电针"委中"穴能促进损伤多裂肌的修复,其机制可能与调节多裂肌铁代谢、减轻组织过氧化损伤相关,并且在电针持续干预3 d后显著促进对铁代谢紊乱水平的调节。     

外泌体miRNA在口腔疾病中的研究进展

外泌体是活细胞分泌至细胞外基质的纳米级囊泡,在细胞之间的物质交换和信号转导方面发挥重要作用,能将其携带的脂质、蛋白质、核酸传递至受体细胞,调节受体细胞的生理、病理过程.微小RNA(microRNA、miRNA)作为外泌体中重要的"货物"被转运至受体细胞,调控受体细胞信号通路,从而对疾病的进展起着调节的作用.近年来,外泌...     

几丁质-硅胶复合膜引导性骨再生的实验研究

目的通过对GBR连续组织学研究及放射学检查,探讨几丁质-硅胶复合膜在引导性骨再生(guided bone regeneration GBR)中的作用.方法取新西兰兔24只,造成双侧桡骨15mm骨缺损,将48只肢体平均分为A、B、C、D组,每组各12只肢体,分别用几丁质-硅胶复合膜、几丁质膜、硅胶管及空白对照.于手术后2、4、6、8、12、16周处死每组各两只兔,标本行X线及组织学检查.结果几丁质-硅胶复合膜组的骨缺损区在成骨活动的活跃程度、骨再生量和再生髓腔结构等方面均优于单纯几丁质、硅胶管及空白对照组(P<     

外固定支架治疗胫腓骨下端波及踝关节的严重粉碎性骨折

目的 探索一种治疗胫腓骨下端波及踝关节严重粉碎性骨折较为理想的方法。方法 应用改良Bastiani单臂多功能外固定支架治疗胫腓骨下端波及踝关节的严重粉碎性骨折47例,跟骨牵引石膏外固定治疗22例,切开复位钢板螺钉内固定治疗17例,并将其术后随访的疗效进行比较。结果 术后随访6个月-3年,平均15个月。根据病人骨折愈合情况,踝关节活动度,关节面平整度及检查患者快速行走200m(100m/min),再登50级楼梯后踝关节疼痛、肿胀及其他不适的严重程度评价疗效。其优良率为：应用外固定支架治疗为74.5%,跟骨牵引石膏外固定治疗为57.1%,切开复位钢板螺钉内理想、固定较牢固、应力遮挡小、手术操作简单、病人可早期下床活动等优点,尤其适用于伴有严重皮肤软组织损伤及缺损的病人。     

脉冲Nd:YAG激光照射牙硬组织后拉力实验

目的研究不同能量和不同频率脉冲Nd:YAG激光照射牙表面后,牙齿与光敏树脂间的拉力大小。方法选择一致性较好的新鲜离体人牙,用高速转头制备各类洞型。然后分别用磷酸腐蚀和不同能量和频率的脉冲Nd:YAG激光进行照射,以光敏树脂进行光固化后做拉力实验并进行比较。结果脉冲Nd:YAG激光刻蚀人牙光固化树脂平均拉力68.9-75.5Kg/cm2,酸腐蚀光固化树脂平均拉力为20.6Kg/cm2,差异有显著意义(P<0.05)。结论用脉冲Nd:YAG激光照射代替磷酸进行牙齿表面处理可增加牙齿与光敏树脂间的粘结力。     

Neutralizing antibody but not hemagglutination antibody provides accurate evaluation for protective immune response to H5N1 avian influenza virus in vaccinated rabbits

In order to develop an animal model and an assay method to evaluate protective immune response to H5N1 avian influenza vaccination, H5N1 avian influenza vaccine was prepared. New Zealand rabbits were assigned to receive two doses of vaccine with different hemagglutinin (HA) dosage. The sera from vaccinated rabbits was evaluated to determine antibody titer and specificity using different tested methods including hemagglutination inhibition assay (HI), neutralizing assay (NT), cross-HI assay, cross-single immunodiffusion assay and cross-neutralization assay. The titer of HI antibody from rabbits immunized with different doses of HA were no less than 1:40 among groups 14 days after the first immunization. Whereas the NT antibody titer was less than 1:10 among groups 14 days after the first immunization. NT antibodies can be detected 14 days after the second immunization in rabbits immunized at HA doses higher than 6 μg, and the NT antibody titers were equal to or higher than 1:40. A good concentration-dependent NT antibody response can be detected in the vaccinated rabbits 14 days after the second immunization, and in contrast, no concentration-dependent relationship can be seen for HA antibody. The cross-HI test showed sera from vaccinated rabbits could cross react with influenza A H5N1 virus with the titers higher than 1:40. No cross reaction among different types (influenza A/H1N1 virus, influenza A/H3N2 virus, influenza B virus and influenza A/H5N1 virus) can be detected in the sera using the single immunodiffusion assay and using NT antibody test. This showed NT antibody test was demonstrated as a more accurate assay method for evaluating vaccination and quality of the vaccine than HI antibody test.     

Foxp3转染对哮喘小鼠脾淋巴细胞功能的影响

目的: 转染Foxp3至哮喘小鼠脾淋巴细胞,探讨Foxp3表达对脾淋巴细胞功能的影响。方法: 卵白蛋白(OVA)致敏激发制作哮喘小鼠模型,收集培养脾脏淋巴细胞;使用电穿孔法转染真核表达载体pcDNA3.1(-)-Foxp3至脾脏淋巴细胞,并设转染空质粒组和对照组;RT-PCR和Western blotting检测Foxp3的表达;流式细胞术检测转染后CD4⁺CD25⁺ Treg细胞/CD4⁺细胞比例;MTT法检测转染后的脾脏淋巴细胞增殖反应,ELISA检测脾淋巴细胞上清中白细胞介素4(IL-4)和干扰素γ(IFN-γ)的含量。结果: 转染组Foxp3 mRNA 和蛋白的表达水平显著高于空质粒组和对照组;转染Foxp3后CD4⁺CD25⁺ Treg细胞/CD4⁺细胞比例显著高于空质粒组和对照组;与空质粒组和对照组相比,转染pcDNA3.1(-)-Foxp3质粒明显抑制了脾淋巴细胞增殖;转染组细胞上清中IL-4和IFN-γ含量低于空质粒组和对照组。结论: 转染pcDNA3.1(-)-Foxp3至哮喘小鼠脾淋巴细胞,Foxp3得到有效表达。Foxp3的高表达能增加CD4⁺CD25⁺ T细胞的数量,抑制哮喘小鼠脾淋巴细胞的增殖以及Th1和Th2细胞因子的产生。     

Association of tumor necrosis factor genetic polymorphism with chronic atrophic gastritis and gastric adenocarcinoma in Chinese Han population

AIM:To investigate the association of TNF polymorphisms with chronic atrophic gastritis (CAG) and gastric adenocarcin-oma in Chinese Han patients.METHODS:The TNFa-e 5 microsatellites and 3 RFLP sites were typed using PCR technique,followed by high-voltage denaturing PAGE with silver staining and restriction enzyme digestion respectively in specimens from 53 patients with CAG and 56 patients with agstric daenocarcinoma and 164 healthy controls.The PCR products were cloned and sequenced.RESULTS:The frequency of TNF-β Ncol*1/2 genotype was higher in patients with chronic atrophic gastritis than in healthy controls,but no significant difference was observed(60.38% vs 46.34%,p=0.076).The frequency of TNa10 allele was significantly higher in patients with chronic atrophic gastritis than in healthy controls(19.81% vs 11.89%,p=0.04).However,it did not relate to age,gender,atrophic degree or intestinal metaplasin in patients with chronic atrophic gastritis,The frequency of TNF-β Ncol*1/2 and d2/d6 genotypes were significantly higher in patients with gastric adenocarcinoma than in healthy indiveduals(p&gt;0.05).However,TNF-β Ncol*1/2 and d2/d6 genotypes did not relate to age,gender,grade of differentiation and clinicopathologic state in patients with gastric adenocarcinoma.The frequency of TNFa6b5c1 hapolotype homoaygote was significantly lower in patients with gastric adenocarcinoma than in healthy controls (1.79% vs 15.85%,p=0.006).CONCLUSION:TNFa10 allele may be a risk factor for chronic atrophic gastritis ,TNF-β Ncol*1/2 and d2/d6 genotypes are associated with the suscepotibility to gastric adenocarcinoma,whereas TNFa6b5c1 haplotype homozygote may contribute to the resistance against gastric adenocarcinoma.